SINGLE AND COMBINED ANALGESIC ACTIVITY TEST OF ETHANOL EXTRACT FROM GUAVA LEAVES (PSIDIUM GUAJAVA L.) AND SOURSOP LEAVES (ANNONA MURICATA L.) IN MALE WHITE RATS INDUCED BY ACETIC ACID

Abstract

ABSTRACT Pain is a disturbing sensory and emotional feeling that is associated with threats, disturbances or tissue damage (Roach, S. S. 2004). The leaves of guava and soursop leaves contain several main compounds such as flavonoids, saponins, and tannins. The purpose of this study was to determine the analgesic activity of the ethanol extract of guava leaves (Psidium guajava L.) and soursop leaves (Annona muricata L.) in male white rats induced by acetic acid.                    This study used 25 male Wistar rats weighing 150 to 200 grams. The rats were randomly divided into 5 groups, the first group receiving 50 mg diclofenac sodium as a positive control group and the second group receiving CMC-Na as a negative control. 0.5%, the third group had a single dose of ethanol extract from guava leaves with a dose of 250 mg / kgBB, the fourth group had a single dose of ethanol extract of 250 mg / kgBB soursop leaves and the fifth group had a combined dose of ethanol extract from guava leaves a dose of 125 mg / kg BB: Ethanol Soursop leaf dose 125 mg / kg body weight. After 30 minutes, 1% acetic acid pain inducer was administered intraperitoneally. Then the amount of stretching was observed for 5 minutes for 1 hour. After the percent strain protection data was obtained, it was analyzed using the Saphiro-Wilk test, the homogeneity test of variants, followed by the Tukey HSD test.                    The results showed that the combined dose of guava leaf ethanol extract at a dose of 125 mg / kgBB: 125 mg / kgBB soursop leaf ethanol showed the optimal percentage of protection and effectiveness compared to the positive control group with a stretch protection of 60.20%. and approached a stretch efficiency of 94. 63%. A single guava leaf with anti-stretch was 47.12% and an elongation efficiency was 74.07%, and a single Soursop leaf with anti-stretch was 48.16% and the stretch efficiency was 75.71%.   Keywords: guava leaves, Psidum guajava L., guava leaves, Annona muricata L. Analgesics.